Standard Operating Procedures In Vitro Toxicology

✍ Scribed by Dr I. P. Sword, Dr Ritchie Thomson (auth.), Dr I. P. Sword, Dr Ritchie Thomson (eds.)

• Publisher: Springer Netherlands
• Year: 1980
• Tongue: English
• Leaves: 255
• Edition: 1
• Category: Library

✦ Synopsis

When Volume 1 (Toxicolpgy) in this series of Standard Operating Procedures was pub­ lished in early 1979, the FDA's Good Laboratory Practice Regulations did not have the force of United States Law, but nevertheless had a substantial impact on the conduct of toxicology in laboratories throughout the world. These Regulations are now in force, and Volume 2 (Pathology) was published later the same year. Our critics have implied that we have attempted to reduce toxicology to the level of the cookery book, or alternatively that we seek to impose our standards on others, In some sinister way ensuring that the IRI code will become the international norm. We dismiss these criticisms as arrant nonsense. The many thousands of volumes already sold worldwide can provide at best a framework for adaptation to suit local laboratory condi­ tions, and thus speed to GLP compliance those organisations which might otherwise have remained foundering at the starting post. If Volumes 1 and 2 of this series have con­ tributed anything to the conduct of toxicology it must surely be in those non-English speaking nations which, because of the international nature of pharmaceutical and chemical trading, are required by commercial pressures to be in compliance with a foreign law formulated in unfamiliar terminology and introduced for reasons that are not immediately obvious. Much has happened in the short period of time since Volumes 1 and 2 were published.

✦ Table of Contents

Front Matter....Pages i-xv
Front Matter....Pages 1-1
SOP/MET/100 General Record Keeping and Archiving Procedures....Pages 5-17
SOP/MET/101 Quality Control Procedures....Pages 19-22
SOP/MET/501 Data Collection and Record Keeping in Analytical Chemistry....Pages 23-27
SOP/MET/502 Archiving Procedures in Analytical Chemistry....Pages 29-31
SOP/MET/503 Procedure for the Receipt and Storage of Drugs and Analytical Standards....Pages 33-35
SOP/MET/504 Procedure for the Receipt and Storage OF Biological Samples Prior to Analysis....Pages 37-39
SOP/MET/505 The Approval of Final Analytical Methods in Analytical Biochemistry....Pages 41-41
SOP/MET/507 Tube Labelling and Data Recording during Pharmacokinetic Studies in Animals....Pages 43-46
SOP/MET/601 Use and Maintenance of Fixed-Volume (Oxford) Pipettes....Pages 47-52
SOP/MET/602 Use and Maintenance of “Class A” Pipettes and Standard Flasks....Pages 53-58
SOP/MET/604 Processing of Analytical Data Derived from Liquid or Gas Chromatograms or from Selected Ion-Monitoring Computer Output....Pages 59-60
SOP/DTX/101 Handling and Safety Procedures for In Vitro Mutagenicity....Pages 63-67
SOP/DTX/102 Handling, Weighing and Disposal of Test Substances in In Vitro Toxicology....Pages 69-73
SOP/DTX/103 Radioactive Materials Storage, Handling and Disposal in In Vitro Toxicology....Pages 75-77
SOP/DTX/104 Microbial Materials — Storage, Handling and Disposal....Pages 79-81
SOP/DTX/105 Tissue Culture Materials Storage, Handling and Disposal....Pages 83-86
SOP/DTX/106 Use of Biological Safety Hoods....Pages 87-90
SOP/DTX/107 Preparation of Liver Homogenates and “S-9” Mix....Pages 91-96
SOP/DTX/108 Quality Control Assay of Liver S-9 Preparations....Pages 97-107
SOP/DTX/109 Recording of Results and Filing within In Vitro Toxicology....Pages 109-111
Front Matter....Pages 1-1
SOP/DTX/110 Use of Autoclaves and Pressure Cookers....Pages 113-115
SOP/DTX/121 Preparation of Media, Quality Control and Storage, for Use in the Ames Test....Pages 117-123
SOP/DTX/122 Sterilisation of Media and Glassware....Pages 125-128
SOP/DTX/123 Ames Assay — Plate Test — and Evaluation of Data Using Salmonella Typhimurium....Pages 129-134
SOP/DTX/124 Ames Assay — Pre-Incubation — Using Salmonella Typhimurium....Pages 135-136
SOP/DTX/125 Ames Assay — Vapour Phase — Using Salmonella Typhimurium....Pages 137-140
SOP/DTX/126 Quality Control of Microbial Cell Lines, Their Maintenance and Storage....Pages 141-146
SOP/DTX/127 Calibration and Use of Biotran Colony Counter....Pages 147-149
SOP/DTX/128 Use of TRP + Reversion in Escherichia Coli (E. Coli WP2, WP2/uvrA − , WP2/uvrA − (PKM101) and WP100)....Pages 151-154
SOP/DTX/129 E. Coli DNA-Repair Plate Assay + Evaluation of Data....Pages 155-156
SOP/DTX/130 E. Coli DNA-Repair Test in Suspension....Pages 157-159
SOP/DTX/132 S. Cerivisiae D5 Recombination Assay and Evaluation of Data....Pages 161-166
SOP/DTX/133 Quality Control of S. Cerevisiae D5, Its Maintenance and Storage....Pages 167-169
SOP/DTX/134 S. Cerevisiae D5 Modified Recombination Assay....Pages 171-174
SOP/DTX/135 Preparation of Media, Quality Control and Storage for Use in E. Coli TRP + Reversion Assay....Pages 175-181
SOP/DTX/136 Preparation of Media, Quality Control and Storage for Use in the E.Coli DNA Repair Assay....Pages 183-189
SOP/DTX/137 Preparation of Media, Quality Control and Storage for Use in S. Cerevisiae D5 Recombination Assay....Pages 191-197
SOP/DTX/141 Preparation of Media Quality Control and Storage-Tissue Culture Cell Maintenance....Pages 199-211
SOP/DTX/143 BHK (Baby Hamster Kidney) Cell Transformation Assay....Pages 213-217
SOP/DTX/144 Gassing and Maintenance of Experimental Dishes....Pages 219-220
Front Matter....Pages 1-1
SOP/DTX/145 Counting of Viability on Plastic Dishes and in Agar....Pages 221-222
SOP/DTX/146 Evaluation of Cell Transformation Data....Pages 223-226
SOP/DTX/147 Mouse Lymphoma Mutation Assay....Pages 227-233
SOP/DTX/148 Evaluation of Mouse Lymphoma Data....Pages 235-238
SOP/DTX/149 Assay of DNA Repair in Human Cells....Pages 239-246
SOP/DTX/150 Assay of DNA Repair Using [ 3 H]-Deoxyguanosine....Pages 247-252
SOP/DTX/152 Assay of DNA Repair by Radioautography....Pages 253-258
SOP/DTX/154 Use of Centrifuge, Ultracentrifuges and Density Gradient Centrifugation....Pages 259-260
SOP/DTX/155 Cytotoxicity Tests....Pages 261-268

✦ Subjects

Laboratory Medicine