In Vitro Methods in Toxicology

✍ Scribed by C. K. Atterwill (editor), C. E. Steele (editor)

• Publisher: Cambridge University Press
• Year: 2009
• Tongue: English
• Leaves: 469
• Category: Library

✦ Synopsis

First published in 1987, this book describes tissue (cell and organ) culture techniques which could be used at a critical predevelopment stage to screen for drug-inducing toxicity or to analyse the molecular mechanisms of toxicity at a later stage in parallel with investigative studies. This type of research has important ethical, financial and scientific implications. In particular, less animals and compound are required in in vitro research and more detailed information at the cellular and subcellular levels can be obtained. The volume brings together the once disparate knowledge of cultural techniques and thus provides an important reference work for industrial and academic toxicologists. It could also form a framework for discussion of potential improvements and modification of toxicity testing.

✦ Table of Contents

Cover......Page 1
Frontmatter......Page 2
Contents......Page 8
List of contributors......Page 10
Preface......Page 12
Introduction......Page 14
TARGET ORGAN TOXICITY......Page 24
In vitro methods in renal toxicology......Page 26
Ototoxicity......Page 50
Cultured cell models for studying problems in cardiac toxicology......Page 71
Use of the isolated rat heart for the detection of cardiotoxic agents......Page 121
Brain reaggregate cultures in neurotoxicological investigations......Page 145
Application of thryoid cell culture to the study of thyrotoxicity......Page 177
In vitro methods to investigate toxic lung disease......Page 201
Metabolism and toxicity of drugs in mammalian hepatocyte culture......Page 222
In vitro evaluation of haemic systems in toxicology......Page 246
GENERAL AND TOPICAL TOXICITY......Page 272
The chorioallantoic membrane in irritancy testing......Page 274
In vitro dermal irritancy tests......Page 290
Genetic toxicology......Page 310
In vitro immunotoxicology......Page 330
REPRODUCTIVE TOXICITY......Page 348
An in vitro test for teratogens using cultures of rat embryo cells......Page 350
Postimplantation embryo culture and its application to problems in teratology......Page 376
Sub-mammalian and sub-vertebrate models in teratogenicity screening......Page 402
The use of in vitro techniques to investigate the action of testicular toxicants......Page 422
An in vitro assessment of ovarian function: a potential tool for investigating mechanisms of toxicity in the ovary......Page 436
CONCLUSION......Page 452
Conclusion......Page 454
Index......Page 458