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In Vitro Refolding of Heterodimeric CapZ Expressed in E. coli as Inclusion Body Protein

โœ Scribed by Kirsten Remmert; Detlef Vullhorst; Horst Hinssen


Book ID
115645552
Publisher
Elsevier Science
Year
2000
Tongue
English
Weight
237 KB
Volume
18
Category
Article
ISSN
1046-5928

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โœ Satoru Misawa; Izumi Kumagai ๐Ÿ“‚ Article ๐Ÿ“… 1999 ๐Ÿ› Wiley (John Wiley & Sons) ๐ŸŒ English โš– 190 KB

Overexpression of cloned or synthetic genes in Escherichia coli often results in the formation of insoluble protein inclusion bodies. Within the last decade, specific methods and strategies have been developed for preparing active recombinant proteins from these inclusion bodies. Usually, the inclus