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Refolding of α1-antitrypsin expressed as inclusion bodies in Escherichia coli: characterization of aggregation

✍ Scribed by Ki-Sun Kwon; Seungcheol Lee; Myeong-Hee Yu

Book ID
113269300
Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
567 KB
Volume
1247
Category
Article
ISSN
0167-4838

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Overexpression of cloned or synthetic genes in Escherichia coli often results in the formation of insoluble protein inclusion bodies. Within the last decade, specific methods and strategies have been developed for preparing active recombinant proteins from these inclusion bodies. Usually, the inclus