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Refolding of G protein α subunits from inclusion bodies expressed in Escherichia coli

✍ Scribed by Emily McCusker; Anne Skaja Robinson

Book ID
116828735
Publisher
Elsevier Science
Year
2008
Tongue
English
Weight
806 KB
Volume
58
Category
Article
ISSN
1046-5928

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Overexpression of cloned or synthetic genes in Escherichia coli often results in the formation of insoluble protein inclusion bodies. Within the last decade, specific methods and strategies have been developed for preparing active recombinant proteins from these inclusion bodies. Usually, the inclus