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Refolding and purification of non-fusion HPT protein expressed in Escherichia coli as inclusion bodies

✍ Scribed by Qin Zhuo; Jian-hua Piao; Rui Wang; Xiao-guang Yang

Book ID: 116828046
Publisher: Elsevier Science
Year: 2005
Tongue: English
Weight: 699 KB
Volume: 41
Category: Article
ISSN: 1046-5928
DOI: 10.1016/j.pep.2004.12.026

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Overexpression of cloned or synthetic genes in Escherichia coli often results in the formation of insoluble protein inclusion bodies. Within the last decade, specific methods and strategies have been developed for preparing active recombinant proteins from these inclusion bodies. Usually, the inclus