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In situ hybridization to somatic metaphase chromosomes of potato

✍ Scribed by R. G. F. Visser; R. Hoekstra; F. R. Leij; L. P. Pijnacker; B. Witholt; W. J. Feenstra

Publisher
Springer
Year
1988
Tongue
English
Weight
721 KB
Volume
76
Category
Article
ISSN
0040-5752

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✦ Synopsis

An in situ hybridization procedure was developed for mitotic potato chromosomes by using a potato 24S rDNA probe. This repetitive sequence hybridized to the nucleolar organizer region (NOR) of chromosome 2 in 95%-100% of the metaphase plates. Another repetitive sequence (P5), isolated from the interdihaploid potato HH578, gave a "ladderpattern" in genomic Southern's of Solanum tuberosum and Solanum phureja, but not in those of Solanum brevidens and two Nicotiana species. This sequence hybridized predominantly on telomeric and centromeric regions of all chromosomes, although chromosomes 7, 8, 10 and 11 were not always labeled clearly.

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