## Identification of phenylthiohydantoins of amino acids-by thin-layer chromatography The, identification of amino acids as their phenylthiohydantoins (PTHs) is of major importance in sequence determinations of peptides and proteins by the EDMAN degradaiion technique. The need for a rapid procedur
Improved thin-layer chromatography technique for the identification of phenylthiohydantoin amino acids
β Scribed by Daniel A. Walz; Jan Reuterby
- Publisher
- Elsevier Science
- Year
- 1975
- Tongue
- English
- Weight
- 313 KB
- Volume
- 104
- Category
- Article
- ISSN
- 1873-3778
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Thin-layer chromatography of PTH amino acids on polyamide sheets with a fluorescent indicator gave a 5&lC%fold increase in sensitivity when compared to thin-layer polyamide plates developed by spray techniques. Resolution of the individual residues in a st.andard mixture remains comparable to that d
Thin-layer chromatogram (6 x 6 cm) of the model mixture: (1) inorganic phosphate, (2) choline, (3) choline phosphate, (4) glycerophosphorylcholine, (5) glycerophosphate, (6) betaine. Solvent systems: First direction (vertical), methanol/water/conc. NH4 OH/chlorotorm/acetone (5 : 2 : 1 : 2 : 2); Seco