## Identification of phenylthiohydantoins of amino acids-by thin-layer chromatography The, identification of amino acids as their phenylthiohydantoins (PTHs) is of major importance in sequence determinations of peptides and proteins by the EDMAN degradaiion technique. The need for a rapid procedur
Miniature thin-layer chromatography of phenylthiohydantoin amino acids application of automatic Edman degradation
β Scribed by M.Cohen Solal; J.L. Bernard
- Publisher
- Elsevier Science
- Year
- 1973
- Tongue
- English
- Weight
- 734 KB
- Volume
- 80
- Category
- Article
- ISSN
- 1873-3778
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Thin-layer chromatography of PTH amino acids on polyamide sheets with a fluorescent indicator gave a 5&lC%fold increase in sensitivity when compared to thin-layer polyamide plates developed by spray techniques. Resolution of the individual residues in a st.andard mixture remains comparable to that d
A simple, rapid, and reliable technique for identifying small amounts of phenylthiohydantoin (PTH) derivatives has been developed for use in the analysis by Edman degradation of small amounts of peptides isolated from fingerprints (10). Solvent systems used in paper and column chromatography of PTH