✦ LIBER ✦

Thin-layer chromatography of sub-nanomole amounts of phenylthiohydantoin (PTH) amino acids on polyamide sheets

✍ Scribed by Murray R. Summers; Gary W. Smythers; Stephen Oroszlan

Publisher: Elsevier Science
Year: 1973
Tongue: English
Weight: 886 KB
Volume: 53
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(73)90114-0

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✦ Synopsis

Thin-layer chromatography of PTH amino acids on polyamide sheets with a fluorescent indicator gave a 5&lC%fold increase in sensitivity when compared to thin-layer polyamide plates developed by spray techniques. Resolution of the individual residues in a st.andard mixture remains comparable to that described previously. Using this technique, the two-dimensional chromatography of each residue from automated sequence analysis is quite feasible even when small amounts of material are available.

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The resolution of a 15-component mixture of phenylthiohydantoin (PTH) amino acids using metal ion impregnated silica gel plates is reported. The spots are located by exposing the chromatograms to an iodine chamber. The method provides a rapid, simple, and less expensive chromatographic system, provi