A simple and fast method for the separation of D- and L-lactic acid enantiomers by thin-layer chromatographic silica gel precoated plates impregnated with Cu(II) is proposed. The two lactic acid enantiomers, dissolved in acetone, are deposed on these plates. D- and L-lactic acids form complexes with
Rapid resolution of phenylthiohydantoin amino acids by thin-layer chromatography on silica gel plates impregnated with transition metal ions
โ Scribed by R. Bhushan; G.P. Reddy
- Publisher
- Elsevier Science
- Year
- 1987
- Tongue
- English
- Weight
- 210 KB
- Volume
- 162
- Category
- Article
- ISSN
- 0003-2697
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โฆ Synopsis
The resolution of a 15-component mixture of phenylthiohydantoin (PTH) amino acids using metal ion impregnated silica gel plates is reported. The spots are located by exposing the chromatograms to an iodine chamber. The method provides a rapid, simple, and less expensive chromatographic system, provides resolution for certain difficult combinations, and leaves the PTH amino acids unaltered chemically.
๐ SIMILAR VOLUMES
## Abstract Cephalosporin antibiotics were separated on thin layer plates impregnated with transition metal ions, Mn^2+^, Fe^2+^, Co^2+^, Ni^2+^ and Cu^2+^, using different concentrations. Various solvent systems were developed for the study and were used for separation of these analytes. Impregnat