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Lactic acid enantiomers: Separation by thin-layer chromatography on silica gel plates impregnated with Cu2+

โœ Scribed by L. Cecchi; P. Malaspina

Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
486 KB
Volume
192
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

A simple and fast method for the separation of D- and L-lactic acid enantiomers by thin-layer chromatographic silica gel precoated plates impregnated with Cu(II) is proposed. The two lactic acid enantiomers, dissolved in acetone, are deposed on these plates. D- and L-lactic acids form complexes with Cu(II), with different Rf values, suitable for radiochemical measurements. The real separation was confirmed by enzymatic tests carried out on spots of D and L forms scraped from the chromatographic plates. Total radioactivity of 14C-labeled D- and L-lactic acids was recovered. A Cu/lactic acid complex is evidenced.

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