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Improved purification and biochemical properties of phosphatidylinositol-specific phospholipase C from Bacillus thuringiensis

✍ Scribed by Thomas KUPKE; Max LECHNER; Georg KAIM; Friedrich GÖTZ


Book ID
115126509
Publisher
John Wiley and Sons
Year
1989
Tongue
English
Weight
502 KB
Volume
185
Category
Article
ISSN
1432-1327

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Phosphatidylinositol-specific phospholipase C was purified in a 27 % yield from the culture medium of Bacillus cereus by a combination of ammonium sulfate precipitation and ion-exchange and hydrophobic interaction chromatography. The purified enzyme was free of other phospholipase C-type activities

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