Improved purification and biochemical properties of phosphatidylinositol-specific phospholipase C from Bacillus thuringiensis
✍ Scribed by Thomas KUPKE; Max LECHNER; Georg KAIM; Friedrich GÖTZ
- Book ID
- 115126509
- Publisher
- John Wiley and Sons
- Year
- 1989
- Tongue
- English
- Weight
- 502 KB
- Volume
- 185
- Category
- Article
- ISSN
- 1432-1327
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Phosphatidylinositol-specific phospholipase C was purified in a 27 % yield from the culture medium of Bacillus cereus by a combination of ammonium sulfate precipitation and ion-exchange and hydrophobic interaction chromatography. The purified enzyme was free of other phospholipase C-type activities
## Abstract A substrain of __Bacillus cereus__ 569/H produced under controlled fermentation conditions in a pilot plant fermentor phospholipase‐C. A partially purified preparation showed good storage stability as a lyophylized powder and in frozen solutions. The preparation contained very small amo