๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Phospholipase-C from Bacillus cereus: Production, purification, and properties

โœ Scribed by J. Shiloach; S. Bauer; I. Vlodavsky; Z. Selinger

Publisher
John Wiley and Sons
Year
1973
Tongue
English
Weight
463 KB
Volume
15
Category
Article
ISSN
0006-3592

No coin nor oath required. For personal study only.

โœฆ Synopsis

Abstract

A substrain of Bacillus cereus 569/H produced under controlled fermentation conditions in a pilot plant fermentor phospholipaseโ€C. A partially purified preparation showed good storage stability as a lyophylized powder and in frozen solutions. The preparation contained very small amounts of phosphomonoesterase and proteolytic activities and essentially no ribonuclease activity. The level of hemolytic activity of the preparation was much lower than that of a commercial preparation of phospholipaseโ€C from Clostridium. Treatment of sarcoplasmic reticulum membrane with phospholipaseโ€C from B. cereus and from Clostridium showed that the B. cereus enzyme caused hydrolysis of 96% of the membrane phospholipids whereas the enzyme from Clostridium could hydrolyze only 80% of the phospholipids.

๐Ÿ“œ SIMILAR VOLUMES

Substrate binding and catalytic mechanis
Substrate binding and catalytic mechanism in phospholipase C from Bacillus Cereus: A molecular mechanics and molecular dynamics study
โœ Dorthe da Graรงa Thrige; Jette R. Byberg Buur; Flemming Steen Jรธrgensen ๐Ÿ“‚ Article ๐Ÿ“… 1997 ๐Ÿ› Wiley (John Wiley & Sons) ๐ŸŒ English โš– 970 KB

For the first time a consistent catalytic mechanism of phospholipase C from Bacillus cereus is reported based on molecular mechanics calculations. We have identified the position of the nucleophilic water molecule, which is directly involved in the hydrolysis of the natural substrate, phosphatidylch

ChemInform Abstract: A Novel Class of Zi
ChemInform Abstract: A Novel Class of Zinc-Binding Inhibitors for the Phosphatidylcholine-Preferring Phospholipase C from Bacillus cereus.
โœ Stephen F. Martin; Bruce C. Follows; Paul J. Hergenrother; Christopher L. Frankl ๐Ÿ“‚ Article ๐Ÿ“… 2000 ๐Ÿ› John Wiley and Sons โš– 30 KB ๐Ÿ‘ 1 views

## Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a โ€œFull Textโ€ option. The original article is trackable v

Purification and properties of a fibrino
Purification and properties of a fibrinolytic enzyme from Bacillus subtilis
โœ Dr. K. I. Fayek; Sanaa T. El-Sayed ๐Ÿ“‚ Article ๐Ÿ“… 2007 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 404 KB

## Abstract A fibrinolytic enzyme obtained from __B. subtilis__ was purified, using DEAEโ€cellulose column chromatography, and gel filtration on Sephadex Gโ€100. The preparation was homogeneous as tested by gel filtration on Sephadex Gโ€200, and disc electrophoresis. The molecular weight of this enzy