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Purification and properties of phosphatidylinositol-specific phospholipase C of Bacillus thuringiensis

โœ Scribed by Taguchi Ryo; Asahi Yoshinari; Ikezawa Hiroh


Book ID
115738099
Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
687 KB
Volume
619
Category
Article
ISSN
0005-2760

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Phosphatidylinositol-specific phospholip
โœ Johannes J. Volwerk; Peter B. Wetherwax; Loreene M. Evans; Andreas Kuppe; O. Hay ๐Ÿ“‚ Article ๐Ÿ“… 1989 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 672 KB

Phosphatidylinositol-specific phospholipase C was purified in a 27 % yield from the culture medium of Bacillus cereus by a combination of ammonium sulfate precipitation and ion-exchange and hydrophobic interaction chromatography. The purified enzyme was free of other phospholipase C-type activities