Functional characteristics of phosphatidylinositol-specific phospholipases C from Bacillus cereus and Bacillus thuringiensis
β Scribed by Johannes J. Volwerk; John A. Koke; Peter B. Wetherwax; O.Hayes Griffith
- Book ID
- 109315033
- Publisher
- John Wiley and Sons
- Year
- 1989
- Tongue
- English
- Weight
- 393 KB
- Volume
- 61
- Category
- Article
- ISSN
- 0378-1097
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Phosphatidylinositol-specific phospholipase C was purified in a 27 % yield from the culture medium of Bacillus cereus by a combination of ammonium sulfate precipitation and ion-exchange and hydrophobic interaction chromatography. The purified enzyme was free of other phospholipase C-type activities
## Abstract A substrain of __Bacillus cereus__ 569/H produced under controlled fermentation conditions in a pilot plant fermentor phospholipaseβC. A partially purified preparation showed good storage stability as a lyophylized powder and in frozen solutions. The preparation contained very small amo