Improved conventional synthesis for 14C-labeled polyglutamates of folic acid

✍ Scribed by Catherine R. Ferenz; David Y. Graham

• Book ID: 102901113
• Publisher: John Wiley and Sons
• Year: 1989
• Tongue: French
• Weight: 562 KB
• Volume: 27
• Category: Article
• ISSN: 0022-2135
• DOI: 10.1002/jlcr.2580270702

✦ Synopsis

form and a r e unable t o support L a c t o b a c i l l u s casei growth u n t i l the 8-linked glutamyls are digested by conjugase enzyues. Most s t u d i e s involving f o l a t e absorption have u t i l i z e d monoglutamyl forms of f o l a t e , p r i m a r i l y f o l i c a c i d .

S y n t h e t i c pteroylpolyglutamates prepared by s o l i d phase o r conllentional

s y n t h e s i s provided conjugated materials with which t o s t u d y the absorption and uetabolism of n a t u r a l d e r i v a t i w s ; h o w w r , t h e s y n t h e t i c hepataglutamates support microbiological growth p r i o r t o enzymatic hydrolysis whereas t h e n a t u r a l conjugates do n o t .

Incomplete p u r i f i c a t i o n of i n t e r m d i a t e peptides during the s y n t h e s i s would be the most l i k e l y explanation of t h i s growth phenomnon. A modified s o l u t i o n s y n t h e s i s has been developed which improws upon intermediate peptide condensations, i n c r e a s e s product y i e l d s , and provides a heptapeptide which does n o t support microbiological growth u n t i l a f t e r enzymatic hydrolysis.