Improved clinical laboratory identification of human pathogenic yeasts by matrix-assisted laser desorption ionization time-of-flight mass spectrometry
✍ Scribed by O. Bader; M. Weig; L. Taverne-Ghadwal; R. Lugert; U. Groß; M. Kuhns
- Book ID
- 111110343
- Publisher
- John Wiley and Sons
- Year
- 2011
- Tongue
- English
- Weight
- 66 KB
- Volume
- 17
- Category
- Article
- ISSN
- 1198-743X
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The proteins isolated from the whole cells of bacterial pathogens and related non-pathogenic ~imulants were analyzed directly, with minimal sample preparation by matrix-assisted laser desorptionlionization (MALDI) tbe-of-flignt mass spectrometry. Inspection of mass spectrometric profiles obtained fr
One problem of matrix-assisted laser desorption ionization coupled to time-of-flight mass spectrometry is the moderate mass accuracy that typically can be obtained in routine applications, Here we report improved mass accuracy for peptides, even when low amounts and complex peptide mixtures are used