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Detection of Pathogenic and Non-pathogenic Bacteria by Matrix-assisted Laser Desorption/ Ionization Time-of-flight Mass Spectrometry

✍ Scribed by T. Krishnamurthy; U. Rajamani; P. L. Ross

Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
622 KB
Volume
10
Category
Article
ISSN
0951-4198

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✦ Synopsis

The proteins isolated from the whole cells of bacterial pathogens and related non-pathogenic ~imulants were analyzed directly, with minimal sample preparation by matrix-assisted laser desorptionlionization (MALDI) tbe-of-flignt mass spectrometry. Inspection of mass spectrometric profiles obtained from direct MALDI-MS analysis of the protein extracts revealed specific biomarkers for individual bacterial cells. The observed biomarkers enabled us not only to detect pathogenic bacteria (Bacillus u n k k , Yersinia pestis and Brucello aeiitensk), but also to distinguish them from the corresponding non-pathogenic species. By examining a series of strains of several BuciZlus species ( u n f h i s , fhuringknsis, cereus and sub&), it was possible to derive genus, species and strain-specific biomarkers from the measured molecular masses of the intact proteins. Additional series of biomarkers were obtained from direct mass spectrometric analysis of tryptic digests of the protein extracts. The application of this technique for rapid chemotaxonomic classi6cation of microorganisms is demonstrated.

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