Matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) was investigated as a method for the rapid identification of whole bacteria, either by comparison with archived reference spectra or by co-analysis with cultures of known bacteria. Bacteria were sampled from c
Identification of bacteria using matrix-assisted laser desorption ionization time-of-flight mass spectrometry
β Scribed by Mollie G. Kedney; Kevin B. Strunk; Lisa M. Giaquinto; Jennifer A. Wagner; Sidney Pollack; Walter A. Patton
- Publisher
- The American Society for Biochemistry and Molecular Biology
- Year
- 2007
- Tongue
- English
- Weight
- 378 KB
- Volume
- 35
- Category
- Article
- ISSN
- 1470-8175
- DOI
- 10.1002/bmb.105
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Several species of the genus Haemophilus are well known etiological agents of pneumonia, meningitis, conjunctivitis, epiglottitis and chancroid. However, identiΓcation and speciation of Haemophilus is both time consuming and labor intensive. Matrix-assisted laser desorption/ionization time-of-Γight
The ionization of nucleosides in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry was systematically investigated using adenine (A), thymine (T), guanine (G) and cytosine (C) with several common matrices. Experimental results of the protonation and deprotonation of the ba
Chemical degradation methods combined with matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) and post-source decay (PSD)-MALDI reflex TOF mass spectrometry (MS) were used to determine the sequence of a peptide branched on to a known peptide backbone. This study was applied to a
The limits of performance of a linear time-of-flight mass spectrometer using matrix-assisted laser desorp tionlionization have been tested. Effects of the pressure in the vacuum system and the sampling rate of the digital oscilloscopes on mass resolution have been investigated. Spectra recorded at d