𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Improved mass accuracy in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of peptides

✍ Scribed by Ole Vorm; Matthias Mann

Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
389 KB
Volume
5
Category
Article
ISSN
1044-0305

No coin nor oath required. For personal study only.

✦ Synopsis

One problem of matrix-assisted laser desorption ionization coupled to time-of-flight mass spectrometry is the moderate mass accuracy that typically can be obtained in routine applications, Here we report improved mass accuracy for peptides, even when low amounts and complex peptide mixtures are used. A new procedure for preparing matrix surfaces is used, and there is no need to mix the matrix with the sample or to add internal standards. Examples are shown with a mass accuracy better than 50 ppm in a peptide mixture. Peptide mapping as well as sequencing by creating "ragged ends" or "ladder sequencing" should benefit especially from the improved mass accuracy.

πŸ“œ SIMILAR VOLUMES

Enhanced mass resolution in matrix-assis
Enhanced mass resolution in matrix-assisted laser desorption/ionization linear time-of-flight mass spectrometry
✍ S. Schuerch; M. Schaer; K. O. Boernsen; U. P. Schlunegger πŸ“‚ Article πŸ“… 1994 πŸ› John Wiley and Sons 🌐 English βš– 465 KB πŸ‘ 2 views

The limits of performance of a linear time-of-flight mass spectrometer using matrix-assisted laser desorp tionlionization have been tested. Effects of the pressure in the vacuum system and the sampling rate of the digital oscilloscopes on mass resolution have been investigated. Spectra recorded at d

Ionization mechanism of oligonucleotides
Ionization mechanism of oligonucleotides in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
✍ Yufei Kong; Yifei Zhu; Jing-Yuan Zhang πŸ“‚ Article πŸ“… 2000 πŸ› John Wiley and Sons 🌐 English βš– 171 KB πŸ‘ 2 views

The ionization of nucleosides in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry was systematically investigated using adenine (A), thymine (T), guanine (G) and cytosine (C) with several common matrices. Experimental results of the protonation and deprotonation of the ba

Sequencing of a branched peptide using m
Sequencing of a branched peptide using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
✍ I. Fournier; P. Chaurand; G. Bolbach; F. LΓΌtzenkirchen; B. Spengler; J. C. Tabet πŸ“‚ Article πŸ“… 2000 πŸ› John Wiley and Sons 🌐 English βš– 163 KB πŸ‘ 2 views

Chemical degradation methods combined with matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) and post-source decay (PSD)-MALDI reflex TOF mass spectrometry (MS) were used to determine the sequence of a peptide branched on to a known peptide backbone. This study was applied to a

Time-of-flight effects in matrix-assiste
Time-of-flight effects in matrix-assisted laser desorption/ionization fourier transform mass spectrometry
✍ T.-P. E. Sze; T.-W. Dominic Chan πŸ“‚ Article πŸ“… 1999 πŸ› John Wiley and Sons 🌐 English βš– 137 KB πŸ‘ 2 views

The time-of-flight effect in Fourier transform mass spectrometry (FTMS) with an external ion source was quantified. The time-of-flight profiles of ions of different masses were measured by stepping the time interval between the ionization pulse and the gating of the trapping voltage (T gate ). The o

Electrospray sample preparation for impr
Electrospray sample preparation for improved quantitation in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
✍ Russell R. Hensel; Richard C. King; Kevin G. Owens πŸ“‚ Article πŸ“… 1997 πŸ› John Wiley and Sons 🌐 English βš– 185 KB πŸ‘ 2 views

The goal of this work is the development of a rapid and objective matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) method for the quantitation of peptides and proteins in human plasma suitable for use in the Good Laboratory Practices (GLP) environment, where