Improved assay of reaction products to quantitate catechol-O-methyltransferase activity by high-performance liquid chromatography with electrochemical detection
✍ Scribed by Ilkka Reenilä; Päivi Tuomainen; Pekka T. Männistö
- Publisher
- Elsevier Science
- Year
- 1995
- Weight
- 406 KB
- Volume
- 663
- Category
- Article
- ISSN
- 0378-4347
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A new assay of catechol O-methyltransferase (EC 2.1.1.6) is described by determination of the W-and p-O-methylated products of 3.4.dihydroxybenzoic acid. The method involves DEAE-Sephadex A-25 chromatography and reversed-phase high-performance liquid chromatography on a LiChrosorb 5 RP 18 column.
A sensitive assay for catechol-O-methyltransferase (COMT) activity by high-performance liquid chromatography with on-line radiochemical detection was described. The method was based on the measurement of 3H-labeled 3-O- and 4-O-methylated products of the substrate, 3,4-dihydroxybenzoic acid, using S