Assay of catechol O-methyltransferase by determination of the m- and p-O-methylated products using high-performance liquid chromatography
β Scribed by Ed J.M. Pennings; Godfried M.J. Van Kempen
- Publisher
- Elsevier Science
- Year
- 1979
- Tongue
- English
- Weight
- 231 KB
- Volume
- 98
- Category
- Article
- ISSN
- 0003-2697
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β¦ Synopsis
A new assay of catechol O-methyltransferase (EC 2.1.1.6) is described by determination of the W-and p-O-methylated products of 3.4.dihydroxybenzoic acid. The method involves DEAE-Sephadex A-25 chromatography and reversed-phase high-performance liquid chromatography on a LiChrosorb 5 RP 18 column.
π SIMILAR VOLUMES
The soluble form of catechol-0-methyltransferase (EC 2.1.1.6.) from rat liver was purified to homogeneity by high-performance anion-exchange chromatography and high-performance gel-filtration chromatography. The specific activity of the final pool was 270 U/mg protein. The purification was 1180-fold
In a study of in vitro and in vivo metabolism of L-3,4dihydroxyphenylalanine (L-Dopa), two methods of high-performance liquid chromatography (HPLC) were used to separate the m-and p-O-methylated products. A reversed-phase column and an aqueous mobile phase by gradient elution were used; the elute w
A sensitive assay for catechol-O-methyltransferase (COMT) activity by high-performance liquid chromatography with on-line radiochemical detection was described. The method was based on the measurement of 3H-labeled 3-O- and 4-O-methylated products of the substrate, 3,4-dihydroxybenzoic acid, using S