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Determination of m- and p-O-methylated products of l-3,4-dihydroxyphenylalanine using high-performance liquid chromatography and electrochemical detection

✍ Scribed by Tadashi Ishimitsu; Shingo Hirose

Publisher: Elsevier Science
Year: 1985
Tongue: English
Weight: 589 KB
Volume: 150
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(85)90514-7

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✦ Synopsis

In a study of in vitro and in vivo metabolism of L-3,4dihydroxyphenylalanine

(L-Dopa), two methods of high-performance liquid chromatography (HPLC) were used to separate the m-and p-O-methylated products. A reversed-phase column and an aqueous mobile phase by gradient elution were used; the elute was analyzed ekctrochemically with a single amperometric and dual coulometric electrode. The L-Dopa and its 0-methylated products could be detected individually in the enzymatic methylation of rat liver homogenate and in patients with Parkinson's disease. Meta/para ratios of O-methylation are easily obtained by this method. Q 1985 Academic PES, IIIC.

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