Simultaneous determination of noradrenaline and 3-methoxy-4-hydroxyphenylglycol in plasma with high-performance liquid chromatography using electrochemical detection

✍ Scribed by Mitsuko Okada; Kazunori Mine; Toshiyuki Noda; Yasufumi Kataoka; Tetsuya Nakagawa

• Publisher: Elsevier Science
• Year: 1988
• Tongue: English
• Weight: 537 KB
• Volume: 175
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(88)90584-2

✦ Synopsis

We herein report the simultaneous determination of the levels of noradrenaline (NA), and 3-methoxy-4-hydroxyphenylglycol (MHPG), a major metabolite of NA. The sample was subjected to a Sep Pak C18 cartridge prior to the NA and MHPG assay by high-performance liquid chromatography with an electrochemical detector. The results correlated well with the established methods. The average percentage of recovery was 91.2 and 98.7% for NA and MHPG, respectively. The intraassay coefficients of variation were 3.7 and 4.6% for NA and MHPG. The interassay coefficients of variation were 3.5 and 7.5% for NA and MHPG, respectively.