Measurement of 3-methoxy-4-hydroxyphenylglycol in human plasma with high-performance liquid chromatography using electrochemical detection

✍ Scribed by Mika Scheinin; Wen-Ho Chang; David C. Jimerson; Markku Linnoila

• Publisher: Elsevier Science
• Year: 1983
• Tongue: English
• Weight: 473 KB
• Volume: 132
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(83)90442-6

✦ Synopsis

A new method utilizing high-performance liquid chromatography with electrochemical detection for the determination of unconjugated 3-methoxy-4-hydroxyphenylglycol, a major metabolite of norepinephrine, in human plasma is described. A novel internal standard, 3-ethoxy-4-hydroxyphenylglycol, was used to correct for variations in extraction efficiency and detector sensitivity. Separation of sample components was achieved isocratically in 20 min per sample in a reversed-phase system. Column temperature had to be carefully regulated. The sensitivity of the assay is suitable for clinical studies, with a limit of detection of 2 pmol/ml. Intra-and interassay precision wasgood, with observed coefficients of variation of 7.5 and 8.88, respectively. Accuracy was tested by comparing the results of the present HPLC method to those found with a specific gas chromatographic-mass spectrometric assay. A satisfactory correlation of 0.96 was obtained, with no significant bias between the two methods.