Measurement of 3-methoxy-4-hydroxyphenylacetic acid (HVA) in plasma by high-performance liquid chromatography with electrochemical detector (HPLC-EC)

✍ Scribed by J.I. Javaid; T.S. Liu; J.W. Maas; J.M. Davis

• Publisher: Elsevier Science
• Year: 1983
• Tongue: English
• Weight: 410 KB
• Volume: 135
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(83)90690-5

✦ Synopsis

A simple and sensitive high-performance liquid chromatography method with electrochemical detector is described for the determination of free 3-methoxy-4-hydroxyphenylacetic acid (HVA) in human plasma. The method does not involve any extraction, is specific and reproducible, and has the potential to measure serotonin (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA) simultaneously. The plasma concentration of free HVA in eight normal, healthy adult volunteers was 10.9 +/- 4.6 (mean +/- SD). In a preliminary study, in one schizophrenic patient the plasma HVA increased twofold after neuroleptic treatment.