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Determination of a new antihypertensive agent (2R,4R)-2-(2-Hydroxyphenyl)-3-(3-mercaptopropionyl)-4-thiazolidinecarboxylic Acid in Blood by High-Performance Liquid Chromatography with Electrochemical Detection

✍ Scribed by Kazutake Shimada; Makoto Tanaka; Toshio Nambara; Yutaka Imai

Publisher
John Wiley and Sons
Year
1984
Tongue
English
Weight
327 KB
Volume
73
Category
Article
ISSN
0022-3549

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✦ Synopsis

A sensitive method has been developed for the determination of a new antihypertensive agent, (2H,4H)-2-(2-hydroxyphenyl)-3-(3mercaptopropionyl)-4-thiazolidinecarboxylic acid (I), in human blood. Because the drug is unstable in biological fluids, it was immediately derivatized by treating the freshly drawn blood specimens with N-(4-ani-1inophenyl)maleimide. The adduct was separated and determined by high-performance liquid chromatography with electrochemical detection o n a reverse-phase column. The assay method was satisfactory with respect to the sensitivity and precision, providing a quantitation limit of ' 2 ng/ml and coefficient of variation of 3%. Preliminary pharmacokinetic data were obtained by orally administering I to two patients with essential hypertension.

Keyphrases 0 Antihypertensive agents-(')R,4H)-2-(2-hydroxyphen-yI)-:{-( :~-mercaptopropionyl)-4-thiazolidinecar~~xylic acid, determination in human blood, high-performance liquid chromatography with elec-

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