Determination of residual 2,4,5-T in vegetables by high performance liquid chromatography with ultraviolet and fluorometric detection after derivatization with 2-(2,3-naphthalimino)ethyl trifluoromethanesulfonate (NE-OTf)
✍ Scribed by Nagata, Tomoko
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 276 KB
- Volume
- 51
- Category
- Article
- ISSN
- 1526-498X
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✦ Synopsis
2,4,5-T was extracted with acetone at below pH 1É0 and the extract was concentrated. After adding 100 g litre~1 sodium chloride solution to the residual solution, 2,4,5-T was extracted with ethyl acetate ] hexane (20 ] 80 by volume). The extract was evaporated to dryness and the residue was dissolved in acetonitrile. 18-crown-6, potassium Ñuoride and NE-OTf were added to the acetonitrile solution and then allowed to react at 50¡C for 20 min. The product was injected to a HPLC with ultraviolet detection operated at 259 nm and Ñuorometric detection at 394 nm emission and 259 nm excitation. The determination limits of the 2,4,5-T derivative in the sample were 20 kg litre~1 with UV detection and 10 kg litre~1 with Ñuorometric detection.