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Measurement of total plasma cysteamine using high-performance liquid chromatography with electrochemical detection

✍ Scribed by Lori A. Smolin; Jerry A. Schneider

Publisher
Elsevier Science
Year
1988
Tongue
English
Weight
477 KB
Volume
168
Category
Article
ISSN
0003-2697

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✦ Synopsis

Cysteamine is currently used to treat children with the inherited disorder nephropathic cystinosis. A method for the quantitative determination of this aminothiol in human plasma is presented. Whole plasma was reduced with sodium borohydride to convert disulfides to thiols. Cysteamine was then separated by high-performance liquid chromatography and detected electrochemically. The recovery of standard cysteamine added to plasma was 96.6 +/- 1.9%. In a patient with cystinosis, an oral dose of cysteamine was absorbed rapidly, with plasma cysteamine reaching a maximum of 56 microM 1 h after the dose. By 1.8 h the plasma cysteamine concentration had decreased to one-half the maximum value.

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