Two water-soluble polymers carrying 0.28 and 0.16 mmol glucose/g were prepared following the co-polymerization of 2-nitro-4-(carboxyhydrazido-N'-acryloyl)benzyl 2,3,4,6-tetra-O-acetyl-/I-n-glucopyranoside and arcylamide. The polymers served as efficient acceptors (up to 86% transfer yield) in the ga
Improved acceptor polymers for enzymic glycosylation
✍ Scribed by Sabine Köpper; Uri Zehavi
- Publisher
- Elsevier Science
- Year
- 1994
- Weight
- 667 KB
- Volume
- 22
- Category
- Article
- ISSN
- 0923-1137
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✦ Synopsis
Different acceptor polymers for enzymic glycosylations were obtained by derivatising cross-linked polyacrylamide beads with various diamines and coupling of glucose via a light-sensitive glycosidic linkage. The carrier-bound glucose was shown to be an effective substrate in enzymic glycosylations with galactosyltransferase. A comparative study of the different acceptor polymers allowed for an evaluation of factors influencing the accessibility of the polymer-bound substrate.
📜 SIMILAR VOLUMES
High-yield methods are presented for the synthesis of D-glycero-D-&ooctulose 8-phosphate and 1 ,&diphosphate, and D-glycero-ido-octulose 1 ,&diphosphate and 8-phosphate. Formate ion-exchange and DEAE-Sephadex A-25 borate column chromatography were used for the isolation of these compounds. A new phe