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Improved methods for the enzymic preparation and chromatography of octulose phsophates

✍ Scribed by Mirek Kapuscinski; Fritz P. Franke; Ian Flanigan; John K. MacLeod; John F. Williams

Publisher
Elsevier Science
Year
1985
Tongue
English
Weight
747 KB
Volume
140
Category
Article
ISSN
0008-6215

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✦ Synopsis

High-yield methods are presented for the synthesis of D-glycero-D-&ooctulose 8-phosphate and 1 ,&diphosphate, and D-glycero-ido-octulose 1 ,&diphosphate and 8-phosphate. Formate ion-exchange and DEAE-Sephadex A-25 borate column chromatography were used for the isolation of these compounds. A new phenylboronate-containing solvent for p.c. and t.1.c. of sugar phosphates is described. The 13C-n.m.r. assignments of the major contributing structure of Dglycero-D-altru-octulose 8-phosphate in aqueous solution (pH 7.0) are presented. *Dedicated to Professor Karl Decker on the occasion of his 60th birthday.

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