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An improved method for the preparation of renaturable apohemoglobin chains

✍ Scribed by Fred K. Friedman; Kenneth Alston; Alan N. Schechter

Publisher: Elsevier Science
Year: 1981
Tongue: English
Weight: 676 KB
Volume: 117
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(81)90698-9

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✦ Synopsis

A rapid, effective procedure for the preparation of renaturable globin chains from human hemoglobin is described. The method utilizes hydrophobic interaction chromatography in an acid medium to separate the a and fi chains of apohemoglobin. Rapid procedures for reconstitution of hemoglobin from these chains and hemin are also presented.

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