A rapid, effective procedure for the preparation of renaturable globin chains from human hemoglobin is described. The method utilizes hydrophobic interaction chromatography in an acid medium to separate the a and fi chains of apohemoglobin. Rapid procedures for reconstitution of hemoglobin from thes
An improved method for the preparation of ‘Mixed-Chain’ phosphatidylethanolamines
✍ Scribed by A. Hermetter; H. Stütz; F. Paltauf
- Publisher
- Elsevier Science
- Year
- 1983
- Tongue
- English
- Weight
- 398 KB
- Volume
- 32
- Category
- Article
- ISSN
- 0009-3084
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A new method is descried for the semisynthetic preparation of mixed-acid phosphatidylethanolamine (PE) having the natural steric configuration. Any phospholipid mixture from natural sources, e,g. soya phospholipids, can be used as the starting material. In the first step, PE is reacted with tritylbr
## Abstract The title method affords the title compound (V) in high purity and is suitable for scale‐up.
Pyridoxal Y-phosphate was synthesized by first oxidizing [Wlpyridoxine to [Wlpyridoxal with MnOp ["C]Pyridoxal, isolated by cation-exchange chromatography, was then reacted with ATP in the presence of rat liver pyridoxal kinase to form ["C]pyridoxal Y-phosphate. The rat liver enzyme was used instead