HepG2 lysates. As expected, the highest signal was found for the liver cell line (24). The proposed assay is highly sensitive and allows screening of high numbers of samples in an easy and rapid way. All these characteristics together make the Determination of Cysteine Residues in Protein test very
Importance of dietary protein level in the carcass analysis method for determining net protein utilisation in the chick
✍ Scribed by H. Fisher; P. Griminger
- Publisher
- John Wiley and Sons
- Year
- 1969
- Tongue
- English
- Weight
- 273 KB
- Volume
- 20
- Category
- Article
- ISSN
- 0022-5142
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A modification of the method of hydroxyproline determination in proteins was devised. The modification consists of the hydrolysis of proteins in 72% perchloric acid of 100°C for 2 hr instead of 20 hr, autoclaving in 6 N HCl or 2 N Ba(OH)\*. Determination of hydroxyproline by the modified method does
Dense and light polyacrylamide gels containing N,N'-( 1,2-dihydroxyethylene)bisacrylamide (DHEBA) or N,N'-diallyltartardiamide (DATD) as the crosslinker have been tested for their solubilization properties. Both types of gel can be dissolved in 10 mM periodic acid. The time and temperature required