## Abstract Abortive transformation of layer cultures of BHK 21 cells by polyoma virus has been studied using serumβdepleted medium. High doses of partially purified infectious virus, in the absence of serum, stimulate the majority of the cells to synthesize DNA while empty capsids do not. The onse
Immunofluorescent tracing of polyoma virus in transformation experiments with BHK 21 cells
β Scribed by Fraser, K.B.; Gharpure, Meera
- Book ID
- 123468183
- Publisher
- Elsevier Science
- Year
- 1962
- Tongue
- English
- Weight
- 767 KB
- Volume
- 18
- Category
- Article
- ISSN
- 0042-6822
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## Abstract When BHK 21 cells are exposed to high multiplicities of polyoma virus, a change in surface antigen may be detected by immunofluorescence, beginning at 18 h and affecting up to 88% of cells 24 h after infection. Timeβlapse cinematography shows that there is a concurrent change in movemen
Some features of the metabolism of untransformed and polyoma-transformed clones of the BHKZI cell strain were studied. I ) No difference in the oxygen uptake of the two groups was detected but in all cases the transformed clones had greater glycolytic capaci!y than the untransformed clones at p H 7.