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Immobilized penicillin V acylase

โœ Scribed by Stina Gestrelius


Book ID
112896287
Publisher
Springer-Verlag
Year
1982
Tongue
English
Weight
127 KB
Volume
7
Category
Article
ISSN
0273-2289

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## Abstract Penicillin acylase has been immobilized to carboxymethylcellulose and to the resin Amberlite XAD7. The reaction kinetics of the enzyme were affected by both intrinsic (molecular) and microenvironmental effects. The Michaelis constant for the enzyme increased after immobilization as a re

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## Penicillin V acylase from jFusarium sp. SKF 235 was i~obilized on several cation-exchange resins, of which Amberlite CG-50 was preferred. Maximum activity of the immobilized penicillin V acylase was 250 to 280 W/g dry beads. The pH and temperature optima of the enzyme shifted from 6.5 to 6.8 a

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Penicillin acylase obtained from E. Coli (E. C. 3.5.1.11) was covalently bound via glutaric aldehyde to acrylic carriers crosslinked with divinylbenzene or ethylene glycol dimethacrylate. The best enzymatic preparation was obtained by using ethyl acrylate/ ethylene glycol dimethacrylate copolymer. I