## Abstract Penicillin acylase has been immobilized to carboxymethylcellulose and to the resin Amberlite XAD7. The reaction kinetics of the enzyme were affected by both intrinsic (molecular) and microenvironmental effects. The Michaelis constant for the enzyme increased after immobilization as a re
Immobilized penicillin V acylase
โ Scribed by Stina Gestrelius
- Book ID
- 112896287
- Publisher
- Springer-Verlag
- Year
- 1982
- Tongue
- English
- Weight
- 127 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0273-2289
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## Penicillin V acylase from jFusarium sp. SKF 235 was i~obilized on several cation-exchange resins, of which Amberlite CG-50 was preferred. Maximum activity of the immobilized penicillin V acylase was 250 to 280 W/g dry beads. The pH and temperature optima of the enzyme shifted from 6.5 to 6.8 a
## Abstract PenicillinโV acylase from the actinomycete __Streptomyces lavendulae__ ATCC 13664 has been immobilized to epoxyโactivated acrylic beads (Eupergit Cยฎ) by covalent binding. Further linkage of bovine serum albumin after enzyme immobilization was carried out in order to remove the remaining
Penicillin acylase obtained from E. Coli (E. C. 3.5.1.11) was covalently bound via glutaric aldehyde to acrylic carriers crosslinked with divinylbenzene or ethylene glycol dimethacrylate. The best enzymatic preparation was obtained by using ethyl acrylate/ ethylene glycol dimethacrylate copolymer. I