Deacylation of benzylpenicillin by immobilized penicillin acylase
β Scribed by Lilly, M.D.; Carleysmith, S.W.; Dunnill, P.
- Book ID
- 122293518
- Publisher
- Elsevier Science
- Year
- 1980
- Tongue
- English
- Weight
- 308 KB
- Volume
- 62
- Category
- Article
- ISSN
- 0300-9084
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## Abstract Penicillin acylase has been immobilized to carboxymethylcellulose and to the resin Amberlite XAD7. The reaction kinetics of the enzyme were affected by both intrinsic (molecular) and microenvironmental effects. The Michaelis constant for the enzyme increased after immobilization as a re
Penicillin acylase obtained from E. Coli (E. C. 3.5.1.11) was covalently bound via glutaric aldehyde to acrylic carriers crosslinked with divinylbenzene or ethylene glycol dimethacrylate. The best enzymatic preparation was obtained by using ethyl acrylate/ ethylene glycol dimethacrylate copolymer. I