✦ LIBER ✦

Immobilization of penicillin acylase on acrylic carriers

✍ Scribed by J. Bryjak; A. Trochimczuk; A. Noworyta

Publisher: Springer
Year: 1989
Tongue: English
Weight: 409 KB
Volume: 4
Category: Article
ISSN: 1615-7605
DOI: 10.1007/bf00369394

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✦ Synopsis

Penicillin acylase obtained from E. Coli (E. C. 3.5.1.11) was covalently bound via glutaric aldehyde to acrylic carriers crosslinked with divinylbenzene or ethylene glycol dimethacrylate. The best enzymatic preparation was obtained by using ethyl acrylate/ ethylene glycol dimethacrylate copolymer. I cm 3 of the carrier bound 6.4 mg of protein, having 72% activity in relation to the native enzyme. The preparation lost only 10% of its initial activity after 100 d of storage at 4~ A negligible effect of immobilization on the enzyme activity at different temperatures or pH as well as significant increase of the stability of the immobilized enzyme at elevated temperatures were observed.

Abbreviations

BA AE PA 6-APA EGDMA DVB butyl acrylate ethyl acrylate penicillin acylase 6-aminopenicillanic acid ethylene glycol dimethacrylate divinylbenzene

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