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Immobilized ferredoxins for affinity chromatography of ferredoxin-dependent enzymes

✍ Scribed by Naoko Sakihama; Kiyoshi Nagai; Hideki Ohmori; Hiroshi Tomizawa; Maki Tsujita; Masateru Shin

Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
723 KB
Volume
597
Category
Article
ISSN
1873-3778

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Several 8-(6aminohexyl)-amino adenine nucleotide derivatives, including ATP, 2',5'-ADP, 3',5'-ADP and desulfo-CoA (CoA, reduced coenzyme A), were prepared and immobilized on Sepharose by cyanogen bromide activation. 8-(6-Aminohexyb-amino-ATP-Sepharose was found to exhibit good aftinity for both NAD+