This work demonstrates that proper selection of a metal ion and chelating ligand enables recovery of a his(6)-tagged protein from canola (Brassica napus) extracts by immobilized metal affinity chromatography (IMAC). When using Co(2+) with iminodiacetate (IDA) as the chelating ligand, beta-glucuronid
A unified partition coefficient theory for chromatography, immobilized enzyme kinetics, and affinity chromatography
✍ Scribed by James B. Taylor; Harold E. Swaisgood
- Publisher
- John Wiley and Sons
- Year
- 1981
- Tongue
- English
- Weight
- 639 KB
- Volume
- 23
- Category
- Article
- ISSN
- 0006-3592
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
An immobilized salicylaldehyde (sal) was used to build various salicylaldehyde-copper-amino acid (Sal-Cu-AA) complexes which are stable at a range of pH values (2.G11.0). The complexes were found to bind protein molecules as IMAC resins. Thirteen proteins were examined for their binding to a Sal-Cu-
## Abstract The avidin/biotin system was applied as a general mediator in the adsorption/desorption or immobilization of biologically active macromolecules to solid supports. In this context, model biotinylated proteins (lectins and antibodies) were attached to avidin‐coupled Sepharose. As examples
## Abstract A sphingosine‐1‐phosphate (S1P) analogue containing a terminal alkyl chain amino group is synthesized in a few steps via olefin cross‐metathesis of an optically resolved intermediate and subsequent phosphorylation. Regioselective acylation of this intermediate at its N terminus in solut