Salicylaldehyde–metal–amino acid ternary complex: A new tool for immobilized metal affinity chromatography

✍ Scribed by D. Leibler; A. Rabinkov; M. Wilchek

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 762 KB
• Volume: 9
• Category: Article
• ISSN: 0952-3499
• DOI: 10.1002/(sici)1099-1352(199634/12)9:5/6<375::aid-jmr268>3.0.co;2-x

✦ Synopsis

An immobilized salicylaldehyde (sal) was used to build various salicylaldehyde-copper-amino acid (Sal-Cu-AA) complexes which are stable at a range of pH values (2.G11.0). The complexes were found to bind protein molecules as IMAC resins. Thirteen proteins were examined for their binding to a Sal-Cu-Gly column. The efficacy of the Sal-CU-AA resin for protein separation were demonstrated by two examples. The first was a new purification process for garlic lectins from garlic crude extract. It seems that in this case the Sal-Cu-AA resins were more selective than IDA resin. The second was immobilization of concanavalin A (Con A) on the resin and using the immobilzed Con A for affinity chromatography of mannose-rich glycoprotein ovalbumin. The Con A could be later eluted with EDTA or imidazole and the Sal-containing polymer could be recharged again for further use.