A sequential method for determination of glucose and fructose involving the u8e of enzymes (hexokinase and glucose-6-phosphate dehydrogenase) immobilized on controlled-pore glass is proposed. The flow is selected so as to determine glucose or both sugars by fluorimetric determination of the NADH for
Immobilized enzyme kinetic study of D-glucose mutarotation by flow injection analysis
β Scribed by Stults, C. L. M.; Wade, A. P.; Crouch, S. R.
- Book ID
- 127214786
- Publisher
- American Chemical Society
- Year
- 1987
- Tongue
- English
- Weight
- 427 KB
- Volume
- 59
- Category
- Article
- ISSN
- 0003-2700
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A method for quantitating glycerophosphorylcholine by flow injection analysis is reported in the present paper. Glycerophosphorylcholine phosphodiesterase and choline oxidase, immobilized on controlled porosity glass beads, are packed in a small reactor inserted in a flow injection manifold. When sa
A flow-mjectlon spectrophotometrx procedure 1s described for the sequential deternunatlon of glucose, fructose and sucrose m a sample solution by usmg s&e and dual lmmobdtzed enzyme reactors m a smgle mamfold Concentrations III the range 10-5-10-3 M are determmed urlth analysis rates of 30-40 determ