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Sequential determination of glucose and fructose in foods by flow-injection analysis with immobilized enzymes

✍ Scribed by P. Linares; M.D.Luque De Castro; M. Valcárcel

Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
412 KB
Volume
202
Category
Article
ISSN
0003-2670

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✦ Synopsis

A sequential method for determination of glucose and fructose involving the u8e of enzymes (hexokinase and glucose-6-phosphate dehydrogenase) immobilized on controlled-pore glass is proposed. The flow is selected so as to determine glucose or both sugars by fluorimetric determination of the NADH formed. The method is applied to the determination of these compounds in fruit juices, yoghourt and dessert powder with good results.

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