A flow-mjectlon spectrophotometrx procedure 1s described for the sequential deternunatlon of glucose, fructose and sucrose m a sample solution by usmg s&e and dual lmmobdtzed enzyme reactors m a smgle mamfold Concentrations III the range 10-5-10-3 M are determmed urlth analysis rates of 30-40 determ
Simultaneous determination of glucose, fructose, and sucrose in mixtures by amperometric flow injection analysis with immobilized enzyme reactors
β Scribed by Matsumoto, Kiyoshi.; Kamikado, Hideaki.; Matsubara, Hiroaki.; Osajima, Yutaka.
- Book ID
- 121663237
- Publisher
- American Chemical Society
- Year
- 1988
- Tongue
- English
- Weight
- 667 KB
- Volume
- 60
- Category
- Article
- ISSN
- 0003-2700
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A sequential method for determination of glucose and fructose involving the u8e of enzymes (hexokinase and glucose-6-phosphate dehydrogenase) immobilized on controlled-pore glass is proposed. The flow is selected so as to determine glucose or both sugars by fluorimetric determination of the NADH for
Flow injection amperometric and chemiluminescence methods for the individual and simultaneous determination of lysine and glucose by using lysine oxidase and glucose oxidase are described in this paper. The calibration graphs for lysine are linear over the range 1 x lOA -1.6x lO-4 and 2.05 x 10W7-2.