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Immobilization of enzymes with polyaziridine: II. D-amino acid oxidase

✍ Scribed by Carrington S. Cobbs; Leon Lantz; Louis L. Wood; Gary J. Calton

Publisher: Springer-Verlag
Year: 1990
Tongue: English
Weight: 345 KB
Volume: 4
Category: Article
ISSN: 0951-208X
DOI: 10.1007/bf00156601

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✦ Synopsis

A novel method of enzyme immobilization using a tri-functional aziridine to immobilize enzymes was used to immobilize D-amino acid oxidase (DAAO) with good retention of enzymatic activity (62%-89%). The stability of the immobilized DAAO in a fixed bed reactor with continuous operation using D-phenylalanine as substrate yielded a projected half-life of 69 days which is far superior to other methods of immobilization of DAAO.

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