Identification of rye chromosomes: the Giemsa banding pattern and the translocation tester set
β Scribed by J. M. Vries; J. Sybenga
- Publisher
- Springer
- Year
- 1976
- Tongue
- English
- Weight
- 869 KB
- Volume
- 48
- Category
- Article
- ISSN
- 0040-5752
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β¦ Synopsis
The Giemsa banding pattern is given for eleven reciprocal translocations of rye, Secale cereale L., together involving all chromosomes at least once, and one telocentric substitution. It is possible to correlate the identification system based on the Giemsa pattern with that based on the translocation tester set. The location of the translocation break points could be determined very exactly for a number of translocations, somewhat less exactly for others. The variations in the banding pattern, resulting from genetic, environmental and technical variation, make definite identification with the nomenclature system of the different rye additions to wheat difficult. An attempt is made, but some caution is necessary.
π SIMILAR VOLUMES
The difficulties encountered in classifying the seven chromosomes of rye (Secale cereale L.) are discussed. Unequivocal classification is possible only with a standard testing system such as a translocation tester set. In the present paper a set is described which contains seven reciprocal transloc
Five subtelocentric primary trisomics of Vicia faba L . were isolated from crosses between asynaptic mutants and normal plants or translocation tester stocks . The extra chromosome of each trisomic was identified cytologically by critical multivalent association at metaphase I of meiosis .
A combination of the silver-staining method of the nucleolus organizer regions (NORs) with a Giemsa-banding method is described. This double staining allows a rapid identification of the NOR-bearing chromosomes.