A simple and rapid technique is described whereby the nucleolus organizer regions (NORs) of human chromosomes can be differentially stained with silver. This staining is followed by trypsin-Giemsa banding on the same metaphase chromosomes. The metaphases simultaneously exhibit silver-stained NORs an
Combined silver staining of the nucleolus organizing regions and Giemsa banding in human chromosomes
โ Scribed by H. Zankl; S. Bernhardt
- Publisher
- Springer
- Year
- 1977
- Tongue
- English
- Weight
- 115 KB
- Volume
- 37
- Category
- Article
- ISSN
- 0340-6717
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โฆ Synopsis
A combination of the silver-staining method of the nucleolus organizer regions (NORs) with a Giemsa-banding method is described. This double staining allows a rapid identification of the NOR-bearing chromosomes.
๐ SIMILAR VOLUMES
The Ag stainability of the nucleolus organizer region (NOR) was studied in the acrocentric chromosomes identified by Q banding of cultured lymphocytes in 41 karyotypically normal persons (33 males and 8 females) originating from southeast Estonia. The data obtained are compared with those establishe
The intensity of the silver staining of nucleolus organizing regions (NORs) and the frequency of chromosomal associations were studied before and after antithyroid treatment in seven and eight hyperthyroid patients, respectively. For one patient, the NOR staining was significantly more intense after
Ag-staining of the nucleolus organizer region (NOR) was studied in the acrocentric chromosomes identified by Q-banding in repeated lymphocyte and skin fibroblast cultures from three different individuals. A similar pattern of Ag-stainability of NORs was found in the two tissues in each individual. S
The Ag-stainability of the nucleolus organizer region (NOR) was studied in the acrocentric chromosomes identified by Q-banding of cultured lymphocytes in 51 karyotypically normal persons (31 males and 20 females). A consistent pattern of Ag-positive NORs was found in each individual. Ninety percent