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A rapid technique for producing silver-stained nucleolus organizer regions and trypsin-Giemsa bands on human chromosomes

✍ Scribed by W. Mike Howell; D. Ann Black

Publisher: Springer
Year: 1978
Tongue: English
Weight: 345 KB
Volume: 43
Category: Article
ISSN: 0340-6717
DOI: 10.1007/bf00396478

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✦ Synopsis

A simple and rapid technique is described whereby the nucleolus organizer regions (NORs) of human chromosomes can be differentially stained with silver. This staining is followed by trypsin-Giemsa banding on the same metaphase chromosomes. The metaphases simultaneously exhibit silver-stained NORs and G bands, allowing for the unequivocal identification of all chromosomes and greatly facilitating studies involving the NOR-bearing acrocentrics.

📜 SIMILAR VOLUMES

Combined silver staining of the nucleolu

Combined silver staining of the nucleolus organizing regions and Giemsa banding in human chromosomes

✍ H. Zankl; S. Bernhardt 📂 Article 📅 1977 🏛 Springer 🌐 English ⚖ 115 KB

A combination of the silver-staining method of the nucleolus organizer regions (NORs) with a Giemsa-banding method is described. This double staining allows a rapid identification of the NOR-bearing chromosomes.