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Identification of metabolites of 4,4′-methylenedianiline in vascular smooth muscle cells by liquid chromatography-electrospray tandem mass spectrometry

✍ Scribed by Kan Chen; Tammy R. Dugas; Richard B. Cole

Book ID
102377821
Publisher
John Wiley and Sons
Year
2006
Tongue
English
Weight
201 KB
Volume
41
Category
Article
ISSN
1076-5174

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✦ Synopsis

Abstract

A capillary liquid chromatography‐electrospray tandem mass spectrometry (LC‐ES‐MS/MS) method was developed for the identification of metabolites of 4,4′‐methylenedianiline (diaminodiphenyl methane, (DAPM)) derived from exposure of vascular smooth muscle cells (VSMC) to this compound. The use of precolumn concentration and column switching techniques prior to reversed‐phase liquid chromatography coupled online to ES‐MS enabled the separation and detection of low‐level DAPM and its metabolites in the exposed cell samples. The employed LC‐ES‐MS method, and further LC‐ES‐MS/MS analysis enabled the structural assignments for two DAPM metabolites from vascular smooth muscle cells: N‐acetyl methylenedianiline (N‐acetyl‐DAPM) and N,N′‐diacetyl methylenedianiline (N,N′‐diacetyl‐DAPM). Copyright © 2006 John Wiley & Sons, Ltd.

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